Aptasensor for Quantification of Leptin Through PCR Amplification of Short DNA-Aptamers

Protein quantification is traditionally performed through enzyme-linked immunosorbent assay (ELISA), which involves long preparation times. To overcome this, new approaches use aptamers as an alternative to antibodies. In this paper, we present a approach quantify proteins with short DNA polymerase chain reaction (PCR) resulting in shorter protocol times comparatively improved limits of detection. The proposed method includes novel way both the target protein and corresponding DNA-aptamers simultaneously, also allows us fully characterize performance aptasensors. Human leptin used validate technique, because it considered important biomarker for obesity-related studies. our experiments, achieved lowest limit detection 100 pg/mL within less than 2 h, affected by dissociation constant aptamer, could be selecting more specific aptamer. Because simple inexpensive approach, technique can employed Lab-On-Chip implementations rapid “on-site” proteins.